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Specific activities and kinetic constants of point-mutated THT-F145Y and THT-FY145,147YF proteins. A, Expression of the mutated THT genes in E. coli and affinity purification of His-tagged mutated THT constructs as described in Figure 7. B, Constructs of point-mutated THT and their specific activities. C, Kinetic constants of the mutated proteins. Enzyme assay and kinetic analysis were performed as described in Figure 7.
