Figure 2.

InsP 5-ptase protein is produced in the transgenic plants and is associated with the PM fraction. A, Crude protein extracts (20 μg protein/lane) from 2-week-old wild type (Wt), vector control (C2), and four independent transgenic lines (2-6, 2-8, 2-17, and 2-12) were analyzed by gel electrophoresis and immunoblotting using an antibody that recognizes the 6-His tag. B, Protein extract from the transgenic line 2-8 was further fractionated by high-speed centrifugation and two-phase partitioning to obtain a PM-enriched fraction (PM), lower phase (LP), and soluble (S) fraction (1 μg protein/lane).