Figure 3.

Roots and hypocotyls of transgenic plants show a reduced gravitropic response. A, Root gravitropic bending of wild type (Wt), vector control (C2), and two InsP 5-ptase transgenic lines (2-6 and 2-8) was monitored over a period of 24 h. Seedlings were grown on vertically oriented plates for 3 d in the light followed by 24 h in the dark. Plates were then rotated by 90° in the dark, and bending was monitored at 6 and 24 h after turning. Bending is plotted as the average from four independent experiments ±se (n = 50 roots/experiment). B, Hypocotyl bending of wild type (Wt), vector control (C2), and three independent transgenic lines (2-6, 2-8, and 2-12) was monitored over 48 h. Seedlings were grown on vertically oriented plates for 3 d in the light followed by 24 h in dark. Plates were then rotated by 90° in the dark and bending monitored 48 h after turning. Bending is plotted as the average from three independent experiments ±se (n = 40 hypocotyls/experiment). The asterisks indicate statistically significant differences between the transgenic lines and the wild type (P < 0.05 using Student's t test).