Table II.
Basal InsP3 content is reduced by approximately 95% in the transgenic lines
InsP3 levels were measured in 7-d-old seedlings grown in the dark. Roots and hypocotyls were harvested separately and pooled from 50 individual seedlings. Wild-type levels are typically in the range of 400 to 600 pmol g−1 fresh weight. The InsP 5-ptase transgenic and vector control InsP3 values are presented as a percentage of the wild type.
| Plant Line
|
InsP3 Contenta
|
|
|---|---|---|
| Root | Hypocotyl | |
| % wild type | ||
| Wild type | 100 | 100 |
| C2 | 107 | 106 |
| 2-6 | 3 | 3 |
| 2-8 | 2 | 2 |
| 2-12 | 9 | 5 |
a
The data presented are the average from two independent experiments.