Table VI.
The development of the asymmetric auxin-induced GUS expression is delayed in the InsP 5-ptase transgenic roots compared with the DR5-GUS control
Histochemical assays and root bending were carried out on DR5-GUS and DR5-GUS InsP 5-ptase roots after 4 and 6 h of bending.
| Time of Bending Lines
|
4 h
|
6 h
|
||
|---|---|---|---|---|
| DR5-GUS | DR5-GUS InsP 5-ptase | DR5-GUS | DR5-GUS InsP 5-ptase | |
| Percent of roots showing gradienta | 66% | 39% | 34% | 61% |
| Average angle of bendingb | 41.9 ± 3.8 | 34.7 ± 2.1 | 52.1 ± 2.9 | 44.0 ± 2.0 |
a
The data presented are the average of two independent experiments (n = 100 roots/line/experiment). The sd was 1% and 6% for the DR5-GUS and DR5-GUS InsP 5-ptase lines, respectively.
b
The data presented are the average of three independent experiments ±sd (n = 12 roots/line/experiment).