Figure 2B.

Thermal stability of Tte-UvrD helicase by ATPase assay. Tte-UvrD was diluted in ThermoPol buffer and incubated at 65°C or 70°C for the indicated time. 2 μl of protein was taken out for ATPase assays performed as described in Materials and Methods. Each reaction consisted of 36 nM Tte-UvrD, incubated at 55°C for 10 min. The absorbance at each temperature was expressed as a percentage of remaining activity relative to the highest absorbance in each assay group. Filled square, incubated at 65°C; open circle, incubated at 70°C. Each data point represents the average of at least three independent experiments. Error bars are means ± S.D.