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The effect of Tte-MutL protein on the unwinding activity of the Tte-UvrD helicase. Helicase activity assays were performed as described in Materials and Methods. Substrate concentration was 0.25 nM, with 0.5 nM Tte-UvrD. Solid column, 3′-ssDNA tailed duplex; open column, blunt-ended duplex; spotted column, 5′-ssDNA tailed duplex. Data points represent the result of a single experiment.
