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. Author manuscript; available in PMC: 2006 Feb 7.
Published in final edited form as: J Biol Chem. 2005 Jun 13;280(32):28952–28958. doi: 10.1074/jbc.M503096200

Figure 8.

Figure 8

Amplification and detection of the pilin invertase homolog gene (pivNg) of Neisseria gonorrhoeae using tHDA. The tHDA reaction was carried out as described in Materials and Methods using a pair of pivNg specific primers and varying amounts of N. gonorrhoeae genomic DNA. 10 μl of the tHDA reaction was separated on a 2% agarose gel and visualized by ethidium bromide staining. The copy numbers of Neisseria gonorrhoeae genomic DNA are: lane 2: 5X105; lane 3: 5X104; lane 4: 5X103; lane 5: 5X102; lane 6: 5X101; lane 7: 0. Lane 1: 200 ng of Low Molecular Weight DNA Ladder (NEB).