FIG. 4.

DNA binding of v-Rel dimerization mutants. v-Rel proteins were translated alone or cotranslated with c-Rel (aa 1 to 284), NF-κB1 (p50), and NF-κB2 (p52) and used in EMSAs with a ³²P-labeled palindromic κB site probe (GGGGAATTCCCC). The Rel/NF-κB proteins in each reaction are indicated on the top of each panel. Reactions with translation mixtures containing v-Rel (A), M1 and M1^M (B), and M3 and M3^R (C) are indicated above each panel. The locations of heterodimeric complexes are indicated by asterisks. All cotranslations contained approximately equal amounts of each protein. The DNA-binding activity of v-Rel, M1, M1^M, M3, and M3^R homodimers when ca. 15-fold more protein is used are shown in panel D.