TABLE 2.

Dimerization and DNA-binding characteristics of v-Rel mutants

Protein	Classa	Dimerizationb with:					DNA bindingc with:
		v-Reld	c-Rel	NF-κB1	NF-κB2	RelA	v-Reld	c-Rel	NF-κB1	NF-κB2
v-Rel		++++	++++	++++	++++	++++	+++	+++	++++	++++
M1	I	+++	++++	+++	++++	−	+/−	++	+++	+++
M2	II	−	+	+++	++++	+	−	+	++	+
M3	III	+	−	++++	++++	−	−	−	++	+
M4	IV	−	−	+	++	+	−	−	+	+
M5	IV	−	−	++++	++++	+	−	−	+	+
M6	V	−	−	+++	++++	−	−	−	++	+
M7	V	−	−	+++	++++	−	−	−	+++	+++
M8	V	−	−	++	++++	−	−	−	+	+
M9	V	−	−	++++	+	−	−	−	+++	+++

^aEach dimerization mutant was assigned to a particular class based on the inability to interact with specific Rel/NF-κB family members.

^bQuantitative liquid β-galactosidase assays were performed to evaluate the formation of homodimers (23.2 ± 1.2) and heterodimers with c-Rel (23.6 ± 1.3), NF-κB1 (135.3 ± 5.1), NF-κB2 (60.7 ± 13.8), and RelA (61.4 ± 1.3). Dimerization: ++++, >75%; +++, 50 to 75%; ++, 25 to 50%; +, <25%; −, none detected. These values represent the β-galactosidase activity relative to that observed for v-Rel.

^cQualitative assessment of DNA-binding activity relative to v-Rel.

^dAssayed for homodimer formation.