TABLE 3.

Transformation of splenic lymphocytes by v-Rel dimerization mutants

Virusa	No. of colonies in soft agarb				Growth in liquidc
	Expt 1	Expt 2	Expt 3	Avg	Expt 1	Expt 2	Expt 3	Avg
CSV	0	0	0	0	0	0	0	0
TW	TMTC	TMTC	TMTC	TMTC	262,144	131,072	131,072	174,763
TM1	36	79	103	73	64	16	64	48
TM2	0	0	0	0	0	0	0	0
TM3	27	0	33	20	32	0	0	11
TM4	0	0	0	0	0	0	0	0
TM5	0	0	0	0	0	0	0	0
TM6	0	0	0	0	0	0	0	0
TM7	0	0	0	0	0	0	0	0

^aHelper virus (CSV) or REV-0-based retroviruses expressing v-Rel (REV-TW) or v-Rel dimerization mutants (REV-TM) were used to infect ConA-stimulated splenic lymphocytes.

^bInfected splenic lymphocytes were plated in soft agar, and colonies were scored microscopically after 10 days.

^cInfected splenic lymphocytes were serially diluted in 96-well plates, and growth was scored microscopically after 10 days. Transformation efficiency was calculated by multiplying the reciprocal of the highest dilution giving visible growth by the number of wells showing growth in that dilution.