FIG. 8.

Northern blot analysis of UL4-CAT or UL4 RNA expression in HFFs infected with RVwt in the presence of the MEK inhibitor UO126. Confluent HFFs were serum starved and pretreated with UO126 for 1 h before infection with RVwt as described in the legend to Fig. 6. The drug was present throughout the experiment. Cytoplasmic RNA was harvested at 8 hpi and analyzed by Northern blot hybridization with either the CAT (A), UL4 (B), or actin (C) probe as described in Materials and Methods. The blot was serially stripped and hybridized with different probes. Lanes: 1, untreated; 2, treated with 10 μM UO126; 3, treated with 20 μM UO126.