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. 2002 May;76(10):5208–5219. doi: 10.1128/JVI.76.10.5208-5219.2002

FIG. 2.

FIG. 2.

(A) Effect of MTX on KSHV DNA replication in uninduced and TPA-induced BC-3 cells. Hybridization was performed with a KSHV GPCR probe. Relative hybridization intensity was standardized to the total DNA hybridization with a human β-globin probe, and the effect of MTX was expressed as a percentage of the untreated control DNA hybridization. Samples: 1, uninduced; 2, TPA induced; 3, uninduced and treated with 1 μM MTX; 4, uninduced and treated with 10 μM MTX; 5, TPA induced for 48 h in the presence of 1 μM MTX; 6, TPA induced for 48 h in the presence of 10 μM MTX; 7, TPA induced and after 24 h treated with 1 μM MTX for an additional 24 h; 8, TPA induced and after 24 h treated with 10 μM MTX for an additional 24 h; 9, TPA induced BJAB. (B) Data representative of three slot blot experiments with three repeats per sample (a, b, and c) for β-globin and viral GPCR (v-GPCR).