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. 2002 May;76(10):5208–5219. doi: 10.1128/JVI.76.10.5208-5219.2002

FIG. 5.

FIG. 5.

(A) RT-PCR of RNA from SV40-BMEC infected with supernatants of BC-3 cells, uninduced, TPA induced, and TPA induced in the presence of 1 μM MTX. Polybrene was used in all samples. Primers specific for ORF73 were used, and amplified fragments were hybridized with an internal probe. Lane 1, SV40-BMEC infected with supernatant of TPA induced BC-3; lane 2, SV40-BMEC infected with supernatant of uninduced BC-3; lane 3, SV40-BMEC infected with supernatant of TPA induced BC-3 in the presence of MTX; lane 4, SV40-BMEC infected with supernatant of BJAB; lane 5, SV40-BMEC without Polybrene. Lanes 6 to 10, same samples as in lanes 1 to 5, respectively, without RT. β-Actin was used as an internal control for cDNA synthesis. (B) Northern blot analysis of RNA extracted from uninduced and TPA induced BC-3 cells after 48 h of treatment with cidofovir (CDV) and foscarnet (PFA) and hybridized with ORF50/Rta, viral GPCR, K12/kaposin, viral IL-6, and human β-actin probes. Samples were loaded in duplicate. Lanes 1 and 2, uninduced; lanes 3 and 4, TPA induced; lanes 5 and 6, BC-3 uninduced and treated with 0.5 μM cidofovir; lane 7 and 8, TPA induced in the presence of 0.5 μM cidofovir; lanes 9 and 10, uninduced and treated with 0.5 mM foscarnet; lanes 11 and 12, TPA induced in the presence of 0.5 mM foscarnet.