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Affinity purification of prokaryotically expressed, His-tagged UL26 protein. Results obtained by 15% PAGE with Coomassie blue stain (A) and Western blot analysis with specific anti-UL26 antiserum (B) and preimmune serum (C) are shown. Lanes: 1, cell lysate from uninduced cells; 2, cell lysate from IPTG-induced cells; 3, affinity-purified protein from IPTG-induced cells. Lane M shows the molecular mass marker, with the sizes indicated on the left.
