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. 2002 May;76(10):4836–4847. doi: 10.1128/JVI.76.10.4836-4847.2002

FIG. 9.

FIG. 9.

Identification of pUL26 as a constituent of the tegument. (A to C) Detection of various HCMV polypeptides by Western blot analysis after detergent treatment of purified virus particles and separation of solubilized and nonsolubilized proteins. (A) Western blot analysis with specific anti-UL26 antiserum; (B) monoclonal antibody 28-77 (7) against the tegument protein pp65; (C) monoclonal antibody 27-156 (46) against the glycoprotein gB. Lanes: 1, insoluble proteins after detergent treatment of virions; 2, solubilized proteins after detergent treatment of virions; 3, complete virions that were used for detergent treatment. (D to F) Western blot analysis of dense bodies and virions. (D) Western blot analysis with specific anti-UL26 antiserum; (E) monoclonal antibody 28-4 (42) against the major capsid protein of HCMV; (F) monoclonal antibody 28-77 against the tegument protein pp65. The sizes of molecular mass markers are shown on the left.