FIG. 2.

Purification and analysis of targeted recombination progeny. (A) Appearance of purified plaques on mouse L2 cells at 72 h postinfection. Alb231 and Alb233 are tiny-plaque recombinants from two independent infection-transfection experiments, and Alb243 is a large-plaque recombinant that arose in the same infection-transfection as Alb231. Alb239 is a wild-type control recombinant reconstructed from fMHV and pMH54 donor RNA. A control mock-infected L2 cell monolayer is included for comparison. (B) RT-PCR analysis. RNA was isolated from infected cells, reverse transcribed with random primer p(N)₆, and amplified with primers PM145 and PM159. PCR products were analyzed by electrophoresis in 1.2% agarose with (+) or without (−) prior digestion with AccI. Lane M, DNA fragment size markers. wt, wild type.