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Reconstruction of viruses containing potential M gene reverting mutations of the MΔ2 lesion. Derivative plasmids were constructed from pLK61, containing the MΔ2 stop codon at codon 227 in addition to a single candidate compensating mutation elsewhere in the M gene. Donor RNAs transcribed from these vectors were used to construct recombinants from fMHV, and the plaque sizes of the resulting double mutants were assessed at 68 h postinfection.
