Table 1.
Thermal stability of m8G-containing G-quadruplexes
| ODN | Tm (°C) | |
|---|---|---|
| d(CGGGGGGTTTTGGGCGGC) | (ODN 1) | 51 |
| d(C(m8G)GGGGGTTTTGGGCGGC) | (ODN 2) | 55 |
| d(CG(m8G)GGGGTTTTGGGCGGC) | (ODN 3) | 45 |
| d(CGGGG(m8G)GTTTTGGGCGGC) | (ODN 4) | 56 |
| d(CGGGGGGTTTT(m8G)GGCGGC) | (ODN 5) | 54 |
| d(CGGGGGGTTTTGGGC(m8G)GC) | (ODN 6) | 54 |
aThe Tms of the oligonucleotides were determined in 2 mM sodium cacodylate buffer (pH 7.0) and 150 mM KCl at 2.5 µM with each strand. SEs were <0.5°C in duplicate determinations.
bThe position of the m8G in ODN 1, ODN 2, ODN 4, ODN 5 and ODN 6 for the syn position; and ODN 3 for the anti position.