Table 1.
Calculated apparent dissociation constants for the M.RsrI–DNA complex and Hill coefficients (nh) for the binding of M.RsrI to DNA of various methylation states [Data for fitting from reference (17)]
| KDapp for monomer binding, nM | KDapp for sequential model, nM | nh for sequential model | |
|---|---|---|---|
| Unmethylated DNA (5 nM) | 15 ± 1.2 | 39 ± 8.8 | 1.3 ± 0.1 |
| Hemimethylated DNA (5 nM) | 4.7 ± 1.0 | 48 ± 7.7 | 2.0 ± 0.1 |
| Hemimethylated DNA (0.5 nM) | 4.8 ± 1.8 | 21 ± 5.0 | 2.2 ± 0.2 |
| Dimethylated DNA (5 nM) | 94 ± 16 | 860 ± 650 | 1.5 ± 0.2 |
The KDapp for the sequential model is the product of the dissociation constant for the binding of the first monomer and the dissociation constant for the binding of the second (25). Model fitting of the sequential and monomer binding models given in the text was performed with EXCEL (Microsoft) and DataFit (Oakdale Engineering) and is shown in Figure1.