. 2006 Feb 6;34(3):806–815. doi: 10.1093/nar/gkj486

Table 2.

Calculated absorbance ratios of M.RsrI, DNA and complex

	ɛ₂₆₀, mM⁻¹ cm⁻¹	ɛ₂₈₀, mM⁻¹ cm⁻¹	A₂₆₀/A₂₈₀
M.RsrI	22.7	48.7	0.47
DNA	336	177	1.90
AdoHcy	15.1	2.11	7.00
1:1::Protein: DNA complex	374	228	1.64
2:1::Protein: DNA complex	412	279	1.48
Observed complex			1.5

Complexes are theoretical 1:1 or 2:1::protein:DNA complexes with 1 bound AdoHcy/protein monomer. A₂₆₀/A₂₈₀ is calculated as the ratio of the molar absorptivities. The molar absorptivities of M.RsrI was calculated according to the method of Gill and von Hippel (18). The molar absorptivities of the oligodeoxyribonucleotide used were calculated from the molecular weight of the DNA, an absorbance of 20 OD/(mg/ml) at 260 nm for double-strand DNA (35), and the observed A₂₆₀/A₂₈₀ ratio. The molar absorptivities of AdoHcy were taken to be equal to that of adenosine (36). The molar absorptivities of complexes were calculated as the sum of the component parts.