Table 2.
Retrotransposition frequency of mouse L1 with mutations to test ORF2 translation
| Construct | % Green fluorescent protein (GFP) |
|---|---|
| TFC | 4.83 ± 0.12 |
| IRES control | 4.70 ± 0.17 |
| ΔIGR | 2.50 ± 0.26 |
| UAA-AUG | 5.80 ± 0.26 |
| AUG-UAA | 2.00 ± 0.10 |
| TFCmut1 | 5.00 ± 0.10 |
IRES control contains a silent substitution in ORF2 that introduces a HpaI site for cloning into TFC element (9), all other constructs are derived from it as indicated by the name: ΔIGR has the IGR between ORF1 and ORF2 removed, juxtaposing the UAA stop of ORF1 with the AUG start of ORF2. UAA-AUG has a stop codon inserted immediately 5′, and AUG-UAA immediately 3′, of the ORF2 AUG. TFCmut1 contains the 3 nt substitutions of mut1 in Figure 7.