FIG. 1.
New World arenavirus infection of mouse ES cells expressing or not expressing α-DG. Virus-specific immunofluorescence staining of mouse ES cells infected with representative viruses of the different clades of the New World arenaviruses. Cells expressing α-DG (A, C, E, and G) are labeled α-DG+/+. α-DG null mutant cells (B, D, F, and H) are labeled α-DG−/−. Viruses: Amapari (A and B) as a clade B representative, Parana (G and H) as a clade A representative, and Latino (C and D) and Oliveros (E and F) as members of clade C. As would be expected, the percentages of virus-positive cells in α-DG+/+ versus α-DG−/− cells per optical field for α-DG-binding viruses, Oliveros and Latino, were 97 and 98%, respectively, and 47 and 52% for the non-α-DG-binding viruses, Amapari and Parana, respectively.