Table 2.
Strong transcriptional activators when fused C-terminally to a Gal4–DBD and expressed in yeast strain with Gal4-binding sites in the His3 reporter gene
| Name | ORF | Process/function | Loc. |
|---|---|---|---|
| ACE2 | YLR131C | Transcription | N |
| ACF2 | YLR144C | Cytoskeleton | S |
| ADR1 | YDR216W | Transcription | N |
| AOS1 | YPR180W | Protein modification | N |
| APC4 | YDR118W | Protein catabolism | N |
| APL2 | YKL135C | Vesicular transport | C |
| ASM4 | YDL088C | Nuclear organization | N,ER |
| ATC1 | YDR184C | Response to stress# | N |
| BCK2 | YER167W | Cell cycle# | C,N |
| BFR2 | YDR299W | Vesicular transport# | N |
| CAK1 | YFL029C | Meiosis,cell cycle | C |
| CHA4 | YLR098C | Transcription | N |
| CRZ1 | YNL027W | Transcription | C,N |
| CSE2 | YNR010W | Transcription | N |
| CUP2 | YGL166W | Transcription | N |
| ECM22 | YLR228C | Transcription | N |
| FCP1 | YMR277W | Transcription | N |
| GAC1 | YOR178C | Meiosis | |
| GAL11 | YOL051W | Transcription | N |
| GLN3 | YER040W | Transcription | N |
| GYP1 | YOR070C | Vesicular transport | G |
| HAP4 | YKL109W | Transcription | N |
| HSF1 | YGL073W | Transcription | N |
| IDS2 | YJL146W | Meiosis# | C,N |
| IKI1 | YHR187W | Transcription | N |
| IME1 | YJR094C | Transcription | N |
| ISF1 | YMR081C | Cellular respiration# | ? |
| LTV1 | YKL143W | Unknown# | C |
| MAS6 | YNR017W | Transport | M |
| MBR1 | YKL093W | Cellular respiration# | ? |
| MCD4 | YKL165C | Protein modification# | S |
| MED4 | YOR174W | Transcription | N |
| MED8 | YBR193C | Transcription | N |
| MET4 | YNL103W | Transcription | N |
| MRS6 | YOR370C | Transport | C |
| MSB4 | YOL112W | Cytoskeleton | S |
| MSN2 | YMR037C | Response to stress | N |
| MVP1 | YMR004W | Transport# | C |
| NAM7 | YMR080C | RNA metabolism | C |
| NAP1 | YKR048C | Budding | C,N |
| NCA3 | YJL116C | Organelle organization# | ? |
| NUP100 | YKL068W | Nuclear organization | N,ER |
| PAT1 | YCR077C | Cell cycle# | C |
| PCL6 | YER059W | Carbohydrate metabolism | |
| PDC2 | YDR081C | Transcription | N |
| PEX19 | YDL065C | Organelle organization# | C |
| PFK27 | YOL136C | Carbohydrate metabolism | C |
| PHO4 | YFR034C | Transcription | C,N |
| PPG1 | YNR032W | Protein modification | C,N |
| PUP2 | YGR253C | Response to stress | N,ER |
| PUT3 | YKL015W | Transcription | N |
| REC114 | YMR133W | DNA metabolism# | N |
| RIM15 | YFL033C | Response to stress | C |
| RLM1 | YPL089C | Cell wall | N |
| ROX3 | YBL093C | Transcription | N |
| RPA12 | YJR063W | Transcription | N |
| RPB3 | YIL021W | Transcription | N |
| RPI1 | YIL119C | Signal transduction | N |
| RPP1A | YDL081C | Protein biosynthesis | C |
| RSP5 | YER125W | Nuclear organization | N |
| RTG1 | YOL067C | Transcription | C,N |
| SCD5 | YOR329C | Vesicular transport | V |
| SCH9 | YHR205W | Protein modification | C |
| SIN4 | YNL236W | Transcription | N |
| SIW14 | YNL032W | Response to stress | C |
| SLA1 | YBL007C | Cell wall | S |
| SNF2 | YOR290C | Transcription | N |
| SPT21 | YMR179W | Transcription# | N |
| SSN2 | YDR443C | Transcription | N |
| STE5 | YDR103W | Signal transduction | V,C,N |
| SWI3 | YJL176C | Transcription | N |
| SWI5 | YDR146C | Transcription | C,N |
| TOA1 | YOR194C | Transcription | N |
| UBP3 | YER151C | Protein modification | C |
| YAL014C | YAL014C | Transport | C |
| YAP1802 | YGR241C | Vesicular transport | S |
| YBL081W | YBL081W | Unknown# | ? |
| YBR061C | YBR061C | Protein biosynthesis | C |
| YBR271W | YBR271W | Unknown | C |
| YCR082W | YCR082W | Unknown# | C,N |
| YDL161w | YDL161W | Vesicular transport | S |
| YDR031w | YDR031W | Unknown# | C,N |
| YDR213W | YDR213W | Transcription | C,N |
| YDR223W | YDR223W | Unknown# | ? |
| YDR260C | YDR260C | Cell cycle# | N |
| YDR291W | YDR291W | Unknown | N |
| YDR320C | YDR320C | Organelle organization# | ER |
| YDR330W | YDR330W | Protein catabolism# | C,N |
| YDR489W | YDR489W | DNA metabolism | N |
| YDR520C | YDR520C | Unknown# | C,N |
| YER045c | YER045C | Transcription | N |
| YER051W | YER051W | Unknown# | ? |
| YGL036W | YGL036W | Unknown# | C |
| YGL079W | YGL079W | Unknown# | C |
| YGL223C | YGL223C | Vesicular transport# | G |
| YGR120C | YGR120C | Vesicular transport | G |
| YGR130C | YGR130C | Unknown# | C |
| YHL012W | YHL012W | Unknown# | ? |
| YHR160C | YHR160C | Transport | P |
| YIL019W | YIL019W | Unknown# | C,N |
| YIL079C | YIL079C | Transport# | N |
| YJL029C | YJL029C | Vesicular transport# | G,C |
| YJL181W | YJL181W | Unknown# | ? |
| YJR070C | YJR070C | Unknown | C,N |
| YJR119C | YJR119C | Unknown# | C,N |
| YKL059C | YKL059C | RNA metabolism# | N |
| YKL171W | YKL171W | Protein catabolism | C |
| YKR060W | YKR060W | Ribosome biogenesis | N |
| YKR064W | YKR064W | Unknown# | C,N |
| YKR077W | YKR077W | Unknown# | C,N |
| YLL013C | YLL013C | RNA metabolism | C |
| YLR135W | YLR135W | DNA metabolism | N |
| YLR192C | YLR192C | Protein biosynthesis | C |
| YLR285W | YLR285W | Vitamin metabolism | C |
| YLR445W | YLR445W | Unknown# | ? |
| YML037C | YML037C | Unknown# | V |
| YMR030W | YMR030W | Cellular respiration# | M,N |
| YMR048W | YMR048W | Cell cycle# | N |
| YMR181C | YMR181C | Unknown# | ? |
| YMR195W | YMR195W | Unknown# | V |
| YMR295C | YMR295C | Unknown# | S |
| YMR299C | YMR299C | Unknown# | C |
| YNR069C | YNR069C | Unknown# | ? |
| YOR066W | YOR066W | Unknown# | ? |
| YOR166C | YOR166C | Unknown# | ? |
| YOR262W | YOR262W | Unknown# | C |
| YPL105C | YPL105C | Unknown# | C |
| YPL229W | YPL229W | Unknown# | C |
| YPL233W | YPL233W | Cell cycle# | N |
| YPL250C | YPL250C | Unknown# | ? |
| YPR008W | YPR008W | Transcription | N |
| YPR179C | YPR179C | Transcription | N |
3AT concentrations of 200 mM or more are required to suppress the amount of His3 expressed in these strains. Process/function abbreviated after GO annotation in SGD, # = molecular function unknown. Localization (Loc.) encoded as nuclear (N), cytoplasmic (C), cytoskeletal/cell wall (S), Golgi (G), endoplasmic reticulum (ER), peroxisome (P), vesicle/membranes (V), mitochondrion (M) or unknown (?).