Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 May;76(10):5131–5139. doi: 10.1128/JVI.76.10.5131-5139.2002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, American Society for Microbiology

PMC Copyright notice

FIG. 2. — Immunohistological analysis of the effects of huTNFR:Fc treatment on FDC status and PrP labeling in spleens of mice already incubating scrapie. (a to d) Light-microscopical analysis. Mice were injected i.p. with scrapie and 38 days later given a single i.p. injection of huTNFR:Fc (b and d) or hu-Ig as a control (a and c). Spleens were obtained 7 days later; frozen sections were stained for FDCs with FDC-M2 monoclonal antiserum (a and b; red), and PrP was detected on paraffin-embedded sections with the PrP-specific polyclonal antiserum 1B3 (c and d; red). Abundant abnormal PrP was detected in association with FDCs in the spleens of control mice 45 days after scrapie injection. However, when mice were treated with huTNFR:Fc 38 days after scrapie injection, heavy PrP labeling was still apparent in the spleen 7 days after treatment despite a temporary absence of FDC-M2 expression. The arrow in panel D indicates a tingible body macrophage containing apoptotic B lymphocytes. Magnification, ×400. (e to i) Ultrastructural analysis. Mice were injected i.p. with scrapie and 38 days later given a single i.p. injection of huTNFR:Fc (g to i) or hu-Ig as a control (e and f). Spleens were obtained 7 days later, and araldite sections were immunostained with the PrP-specific polyclonal antiserum 1A8. (e) An area of immunogold reactivity (indicated with an asterisk) is present between lymphocytes in the germinal center of a scrapie-injected, hu-Ig-treated control mouse. This focal immunoreaction is associated with a complex knot of mature FDC processes. Part of a tingible body macrophage is present at the bottom left (arrow). Bar = 1.76 μm. (f) High magnification of the FDC complex indicated with an asterisk in panel e. Highly convoluted FDC processes are associated with the immunogold reaction. Many short linear and curvilinear structures are immunolabeled. Bar = 0.35 μm. (g) Edge of a germinal center showing marked apoptosis (black arrows) in the spleen of a scrapie-injected, huTNFR:Fc-treated mouse. Immature FDC dendritic processes surround the germinal center (white arrows). Bar = 1.87 μm. (h) Tingible body macrophage (arrow) in the spleen of a scrapie-injected, huTNFR:Fc-treated mouse showing intralysosomal PrP accumulation. Moderately reactive FDC dendrites lie adjacent to the macrophage. Bar = 1.31 μm. (i) Foci of immunoreactivity associated with immature FDC dendritic processes in the spleen of a scrapie-injected, huTNFR:Fc-treated mouse. The immunogold reaction is associated with electron-dense material in the extracellular space surrounding FDC dendrites. Bar = 0.56 μm.

FIG. 2. — Immunohistological analysis of the effects of huTNFR:Fc treatment on FDC status and PrP labeling in spleens of mice already incubating scrapie. (a to d) Light-microscopical analysis. Mice were injected i.p. with scrapie and 38 days later given a single i.p. injection of huTNFR:Fc (b and d) or hu-Ig as a control (a and c). Spleens were obtained 7 days later; frozen sections were stained for FDCs with FDC-M2 monoclonal antiserum (a and b; red), and PrP was detected on paraffin-embedded sections with the PrP-specific polyclonal antiserum 1B3 (c and d; red). Abundant abnormal PrP was detected in association with FDCs in the spleens of control mice 45 days after scrapie injection. However, when mice were treated with huTNFR:Fc 38 days after scrapie injection, heavy PrP labeling was still apparent in the spleen 7 days after treatment despite a temporary absence of FDC-M2 expression. The arrow in panel D indicates a tingible body macrophage containing apoptotic B lymphocytes. Magnification, ×400. (e to i) Ultrastructural analysis. Mice were injected i.p. with scrapie and 38 days later given a single i.p. injection of huTNFR:Fc (g to i) or hu-Ig as a control (e and f). Spleens were obtained 7 days later, and araldite sections were immunostained with the PrP-specific polyclonal antiserum 1A8. (e) An area of immunogold reactivity (indicated with an asterisk) is present between lymphocytes in the germinal center of a scrapie-injected, hu-Ig-treated control mouse. This focal immunoreaction is associated with a complex knot of mature FDC processes. Part of a tingible body macrophage is present at the bottom left (arrow). Bar = 1.76 μm. (f) High magnification of the FDC complex indicated with an asterisk in panel e. Highly convoluted FDC processes are associated with the immunogold reaction. Many short linear and curvilinear structures are immunolabeled. Bar = 0.35 μm. (g) Edge of a germinal center showing marked apoptosis (black arrows) in the spleen of a scrapie-injected, huTNFR:Fc-treated mouse. Immature FDC dendritic processes surround the germinal center (white arrows). Bar = 1.87 μm. (h) Tingible body macrophage (arrow) in the spleen of a scrapie-injected, huTNFR:Fc-treated mouse showing intralysosomal PrP accumulation. Moderately reactive FDC dendrites lie adjacent to the macrophage. Bar = 1.31 μm. (i) Foci of immunoreactivity associated with immature FDC dendritic processes in the spleen of a scrapie-injected, huTNFR:Fc-treated mouse. The immunogold reaction is associated with electron-dense material in the extracellular space surrounding FDC dendrites. Bar = 0.56 μm.