FIG. 3.
Blockade of the TNFR signaling pathway significantly extends the incubation period of disease following peripheral injection with ME7 scrapie. (a) Mice were treated with huTNFR:Fc (□) or hu-Ig as a control (▪) 5 days before or 14 days after i.c. injection with a moderate dose of scrapie (1.0% [wt/vol] scrapie brain homogenate). (b) Mice were treated with huTNFR:Fc (□) or hu-Ig as a control (▪) 5 days before i.p. injection with a moderate (1.0%) or limiting (0.1% or 0.01%) dose of scrapie. ○, incubation periods for individual mice. (c) Mice were treated with huTNFR:Fc (□) or hu-Ig (▪) 5 days before or 14 or 38 days after i.p. injection with a moderate dose of scrapie. Each bar represents a mean ± standard error of the mean for six to nine mice. The vertical broken line represents the time at which two spleens were taken from each treatment group for subsequent analysis of scrapie infectivity and PrPSc accumulation (see Fig. 4). d, days.