Skip to main content
. 2002 May;76(10):5014–5023. doi: 10.1128/JVI.76.10.5014-5023.2002

FIG. 1.

FIG. 1.

3′ end of the HPV-11 LCR (P93 promoter), structures of fluorescein-labeled oligonucleotides TATASITE, E2TATA, and E1E2TATA, and description of pUC18/7870-99. (A) The phosphoramidite-linked fluorescein moiety structure is illustrated. (B) The sequence of the 3′ end of the HPV-11 LCR and the positions of the E1, E2, and TBP binding sites are illustrated, as well as oligonucleotides TATASITE, E2TATA, and E1E2TATA. The sequence of TATASITE contains the full TATA box and its flanking bases, nucleotides 58 to 75. Oligonucleotide E2TATA, nucleotides 45 to 89, contains the TATA box and the adjacent E2 site. Oligonucleotide E1E2TATA, nucleotides 7908 to 98, contains the E1, two E2, and TBP binding sites. The plasmid pUC18/7870-99 contains nucleotides 7870 to 99 with the E1, three E2, and TBP binding sites ligated into pUC18. Note that sequence numbering begins at the origin in the E1 binding site.