TABLE 2.
PERCENTAGE OF BOUND γ-CRYSTALLINS TO α-CRYSTALLINS UNDER EQUILIBRIUM CONDITIONS
Peak | Percent bound |
---|---|
S300-5 | 14 ± 1 |
S300-7 | 18 ± 8 |
S300-8 | 10 ± 2 |
The initial concentrations were 40 μM α-crystallin and 50 μM γ-crystallin, which correspond to a weight ratio of 4:5, respectively. The calculation for each peak is the average of three determinations and the standard deviation, calculated from the weight of γ-crystallin bound to the same weight of α-crystallin.