TABLE 2.
Comparison of percentage of PBMC expressing p24-Ag to number of viral gag copies per 100 cellsa
| Virus | Virus dilution | % of cells positive for p24-Ag | No. of copies of gag DNA per 100 PBMC | Fold difference |
|---|---|---|---|---|
| BaL | — | 2.83 | 8.97 | 3.17 |
| 1:2 | 2.05 | 7.40 | 3.61 | |
| 1:4 | 1.74 | 6.73 | 3.87 | |
| 1:8 | 1.14 | 4.20 | 3.69 | |
| 1:16 | 0.85 | 2.47 | 2.91 | |
| 1:32 | 0.48 | 1.64 | 3.42 | |
| SF162 | — | 3.89 | 18.11 | 4.66 |
| 1:2 | 2.30 | 13.28 | 5.78 | |
| 1:4 | 1.46 | 6.97 | 4.78 | |
| 1:8 | 0.94 | 5.36 | 5.71 | |
| 1:16 | 0.52 | 2.78 | 5.36 | |
| 1:32 | 0.24 | 1.40 | 5.88 |
a
PHA- and IL-2-stimulated PBMC were exposed to serial dilutions of virus in medium containing indinavir. On day 2, aliquots were split and evaluated by flow cytometry for intracellular p24-Ag expression and by quantitative PCR for viral gag DNA. The MOI of undiluted virus was ∼1.0.