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The interaction between Upf1 and eRF3 increased upon overexpression of His-SMG-1-DA. HeLa TetOff cell lysates transfected with the plasmids shown above. Cell lysates (input) were immunoprecipitated with the antibodies under the conditions of RNase-treatment as shown on the top and were probed with the antibodies shown on the left. Input represents 10% of the amount immunoprecipitated.
