Table 1.
Crossover areas identified in three donors from the 2.5-Mb region
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Crossovers
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|---|---|---|---|---|---|---|---|---|---|---|
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Region
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Observed
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| Donor | Name | Start (bp) | End (bp) | Size (bp) | Number | Rate (cM) | Relative rate (cM/Mb-1) | Expected ratea(cM) | Observed/expected | Probability of having observed crossovers or moreb |
| D-11 | X1 | 38152612 | 38178652 | 26,040 | 1 | 0.2119 | 8.14 | 0.0645 | 3.28 | – |
| X2 | 38218211 | 38266729 | 48,518 | 5 | 1.0593 | 21.83 | 0.1202 | 8.81 | 3.0123E-04 | |
| X3 | 38628305 | 38636185 | 7880 | 2 | 0.4237 | 53.77 | 0.0195 | 21.71 | 3.9856E-03 | |
| X4 | 38739959 | 38758968 | 19,009 | 1 | 0.2119 | 11.15 | 0.0471 | 4.50 | – | |
| X5 | 38797827 | 38811501 | 13,674 | 1 | 0.2119 | 15.49 | 0.0339 | 6.25 | – | |
| X6 | 39032715 | 39093924 | 61,209 | 1 | 0.2119 | 3.46 | 0.1516 | 1.40 | – | |
| X7 | 39262278 | 39272176 | 9898 | 1 | 0.2119 | 21.40 | 0.0245 | 8.64 | – | |
| X8 | 39628371 | 39934305 | 305,934 | 3 | 0.6356 | 2.08 | 0.7579 | 0.84 | 6.9429E-01 | |
| X9 | 39945953 | 39948593 | 2640 | 1 | 0.2119 | 80.25 | 0.0065 | 32.39 | – | |
| X10 | 39991122 | 40023327 | 32,205 | 2 | 0.4237 | 13.16 | 0.0798 | 5.31 | 5.5319E-02 | |
| X11 | 40168288 | 40189134 | 20,846 | 1 | 0.2119 | 10.16 | 0.0516 | 4.10 | – | |
| X12 | 40196686 | 40202975 | 6289 | 5 | 1.0593 | 168.44 | 0.0156 | 67.99 | 1.6513E-08 | |
| X13 | 40237524 | 40248168 | 10,644 | 1 | 0.2119 | 19.90 | 0.0264 | 8.03 | – | |
| X14 | 40291433 | 40302607 | 11,174 | 1 | 0.2119 | 18.96 | 0.0277 | 7.65 | – | |
| X15 | 40302607 | 40338244 | 35,637 | 1 | 0.2119 | 5.95 | 0.0883 | 2.40 | – | |
| X16 | 40461817 | 40466449 | 4632 | 3 | 0.6356 | 137.22 | 0.0115 | 55.39 | 2.5274E-05 | |
| Total | 616,229 | 30 | 6.3559 | 10.31 | 1.5266 | 4.16 | – | |||
| D-12 | X17 | 38155214 | 38250530 | 95,316 | 2 | 1.7391 | 18.25 | 0.2361 | 7.37 | 3.0650E-02 |
| X18 | 38250530 | 38266729 | 16,199 | 1 | 0.8696 | 53.68 | 0.0401 | 21.67 | – | |
| X19 | 39747011 | 39768010 | 20,999 | 1 | 0.8696 | 41.41 | 0.0520 | 16.72 | – | |
| X20 | 40298992 | 40311185 | 12,193 | 1 | 0.8696 | 71.32 | 0.0302 | 28.79 | – | |
| X21 | 40368736 | 40467182 | 98,446 | 1 | 0.8696 | 8.83 | 0.2439 | 3.57 | – | |
| Total | 243,153 | 6 | 5.2174 | 21.46 | 0.6024 | 8.66 | – | |||
| D-8 | X22 | 38202979 | 38250530 | 47,551 | 3 | 4.0000 | 84.12 | 0.1178 | 33.96 | 1.0359E-04 |
| X23 | 38739959 | 38761795 | 21,836 | 1 | 1.3333 | 61.06 | 0.0541 | 24.65 | – | |
| X24 | 38985699 | 39039429 | 53,730 | 1 | 1.3333 | 24.82 | 0.1331 | 10.02 | – | |
| Total | 123,117 | 5 | 6.6667 | 54.15 | 0.3050 | 21.86 | – | |||
| Total/Average | – | – | 1,964,998 | 41 | 6.1934 | 2.48 | – | – | – | |
a
Values in this column are expected if the crossovers were evenly distributed in the 2.5-Mb region
b
Calculated by the binomial distribution test. P-values are the probability for the observed or more to occur and therefore to constitute a recombination hotspot. Because single crossovers cannot constitute any statistic power, no probability of occurrence can be calculated