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HeLa cells were transfected with pCi-MHV-N (A and B [except inset panel]), fixed, and analyzed by indirect immunofluorescence with rabbit anti-MHV polyclonal sera (red). The nucleolus was detected with anti-nucleolin (human) mouse monoclonal antibody (green). (A) Colocalization when it occurs is shown in yellow and is denoted by an arrow. (B) Inset panel is from mock-transfected HeLa cells. Magnifications: A and B, ×15 (resolved an additional four times) and ×60, respectively.
