FIG. 7.

Vero cells were transfected with pFibrillarin-GFP and pCi-MHV-N, fixed, and analyzed by indirect immunofluorescence with rabbit anti-MHV (red) and direct fluorescence to visualize GFP. Differentially fluorescing images were gathered separately from the same 0.5-μm-thick optical section by using a confocal microscope and the appropriate filter. Two pairs of images (A+B and D+E) were digitally superimposed to depict the distribution of MHV N protein and fibrillarin-GFP fusion protein (C and F, respectively). Magnification, ×60 (all panels).