FIG. 5.

Localization patterns of the truncated VP22 proteins in COS-1 cells arrested in mitosis. Expression vectors for GFP, full-length VP22, and the truncated VP22s were transfected into COS-1 cells grown in coverslip chambers. Twenty-four hours later nocodazole was added to the media, and cells were incubated for a further 16 h. Live expressing cells were then analyzed by confocal microscopy, and representative images were acquired. Wt, wild type.