FIG. 6.
The gB retrieval from the cell surface is not necessary for the production of normal levels of infectious virus progeny. U373 tTa were coinfected with HCMV (MOI = 10) and either the adenovirus expressing dynWT or dynK44A (MOI = 10). Cell lysates and supernatants were prepared from the coinfected U373 cells at either day 1 or day 5 p.i. and then assayed for the production of cell-associated or extracellular virus using a standard plaque assay. Inhibition of gB endocytosis did not affect the levels of infectious virus produced in cell-associated or supernatant fractions. These results demonstrate that, although gB can be retrieved from the cell surface for incorporation within the virion, this pathway is not necessary for the production of normal levels of infectious virus progeny.