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. 2026 Sep 14;17:1826616. doi: 10.3389/fpls.2026.1826616

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Copyright © 2026 Buffagni, Danzi, Ramirez-Gonzalez, Uauy, Gullì, Simmonds, Petrozza, Cellini, Summerer, Pignone, Janni and Marmiroli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Four-panel scientific workflow diagram depicting DNA extraction from wheat, PCR amplification of two genes, pooling of 190 PCR samples into four groups, sequencing with Illumina NovaSeq6000 for SNP identification, and assigning SNPs to genotypes using KASP for biallelic SNPs. — Scheme of the method used for the allele mining. (A) Targeted enrichment by PCR is followed by (B) a two-step pooling to reduce the number of samples for the (C) sequencing by NGS. After the alignments of all the reads to the reference sequence, the identified SNPs are finally (D) assigned to the corresponding genotype with a KASP assay.