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. 2002 May;76(10):4971–4986. doi: 10.1128/JVI.76.10.4971-4986.2002

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Copyright © 2002, American Society for Microbiology

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FIG. 1. — Determination of optimal ratio of PBMC to supplemental recombinant vaccinia virus-infected autologous BLCL for maximizing the sensitivity of IFN-γ-producing cell detection. Vertical bars represent the SFU/10⁶ PBMC for control wells (vP1170, vaccinia virus WR; stippled bars) and test wells (vP1288, expressing the IIIB pol gene; solid bars) performed in duplicate. (A) Cryopreserved PBMC thawed and rested overnight (as outlined in Results) prior to addition to assay. (B) Cryopreserved PBMC added directly to assay postthaw.