FIG. 10.

HLA restriction analysis of the gag-specific cross-subtype CD8 response of subject VAIP-4 mapped to peptide YVDRFYKTL (gag 296-304). Effector cells were generated by 20-mer peptide stimulation (peptide 30 from 92UG037 gag) of cryopreserved PBMC, and target cells were pulsed overnight with peptide at 10 μg/ml. Percent specific killing of autologous and partially HLA class I-matched BLCL is shown at E:T ratios of 50:1 (solid bars), 25:1 (Z-hatched bars), 10:1 (S-hatched bars), and 2:1 (stippled bars). The HLA class I type of each target cell is shown along the x axis.