FIG. 6.

Cytotoxic activity of peptide-stimulated cultures of cryopreserved PBMC. The identified immunodominant 20-mer peptides were used to generate effector cells from subjects AIHP-6 (A), VAIP-4 (B), and US101 (C). Vaccinia virus-infected autologous BLCL served as target cells. Percent specific killing is shown for target cells infected with the gag constructs for AIHP-6 and VAIP-4 and the env constructs for US101 at E:T ratios of 50:1 to 2:1. Autologous BLCL infected with vSC8 served as the negative controls.