FIG. 4.

Capsid assembly kinetics show evidence for both inhibition and misdirection by bis-ANS. (A) In assembly at a low level of ionic strength (0.25 M NaCl), increased light scatter observed with low concentrations of bis-ANS suggests changes in assembly path and products. Samples contained 10 μM Cp149₂ with various bis-ANS concentrations. (B) At a high level of ionic strength (0.5 M NaCl), bis-ANS showed only inhibitory effects. Kinetics of capsid assembly as induced by NaCl were observed in real time by monitoring light scattering. In the absence of bis-ANS, there was an excellent correlation between light scattering and capsid concentration (35).