FIG. 7.

Replication kinetics of HAV5 (A), HAV5-ΔE1 (B), BAV3 (C), or BAV3-ΔE1A (D) in hybrid cell lines. 293-Puro, MDBK-Neo, BHH2C, BHH3, or BHH8 cell monolayers in 60-mm dishes were infected with HAV5, HAV5-ΔE1 (AdCA36lacZ), BAV3, or BAV3-ΔE1A (BAV3ΔE1AE3) at a multiplicity of infection of 2 PFU per cell. After virus adsorption, virus-infected cell monolayers were washed with PBS and incubated at 37°C in MEM containing 2% FetalClone III. At 6, 24, 48, and 72 h postinfection, cells were harvested along with the medium and freeze-thawed three times, and virus titrations were done by plaque assays.