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Transfection efficiency of BHH cell lines. 293-Puro, MDBK-Neo, BHH2C, BHH3, or BHH8 cell monolayers in 60-mm dishes were transfected with 5 μg of PacI-digested pMvOBAV3ΔE1lacZ by using Lipofectin-mediated transfection protocol (Life Technologies). At 48 h posttransfection, the cells were prepared for LacZ histochemistry (A) or LacZ assay (B). The assays were done in triplicate, and the results are presented as mean ± the standard deviation.
