FIG. 2.

Intracellular cytokine analyses with Gag peptide pools in high-dose Gag-Pol-vaccinated animals at 1 week after administration of the rMVA booster. PBMC were stimulated with four Gag pools (22-mers overlapping by 12 amino acids) for 6 h, fixed, and stained for CD3, CD8, and IFN-γ. Cells were gated on lymphocytes, followed by CD3, and analyzed for CD8 and IFN-γ. Cells in the left quadrants represent CD4⁺ (CD3⁺ CD8⁻). The frequencies in the upper quadrants are IFN-γ-producing cells as percentages of the total number of CD4⁺ (left quadrants) or CD8⁺ (right quadrants) cells.