TABLE 2.
HIV-1 p24 and CMV antigen-specific CD4+ T-cell expansions from HIV-1-infected donors
| Donor | Peripheral CD4+ T-cell count (cells/mm3) | Copies of HIV-1 RNA/ml of plasma | HIV-1 p24 stimulation
|
CMV stimulation
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| % IFN-γ+ CD4+ T cells at daya:
|
Expansionb | Copies of HIV-1 RNAc | % IFN-γ+ CD4+ T cells at day:
|
Expansion | Copies of HIV-1 RNA | |||||
| 0 | 8 | 0 | 8 | |||||||
| UH32 | 826 | 32,681 | 0.02 | 0 | 0 | 5,724 | 0.09 | 2.2 | 24.4 | 39,404 |
| UH90 | 48 | 153,713 | 0.1 | 0 | 0 | 3,314 | NDd | 0 | 0 | 3,154 |
| UH91 | 107 | 4,993 | 0.07 | 0 | 0 | 1,380 | 0.49 | 12.08 | 24.6 | <200 |
| UH20 | 1,273 | <20 | 0.04 | 4.53 | 113 | 8,576 | 3.75 | 7.44 | 1.9 | <200 |
| UH8 | 523 | <20 | 0.04 | 4.08 | 102 | ND | 0.1 | 0 | 0 | ND |
| UH52 | 667 | <20 | 0.03 | 3.80 | 100 | 502 | 0.66 | 13.31 | 20.1 | 200-400 |
| UH64 | 942 | <20 | 0.04 | 0.67 | 16.7 | 2,429 | 0.16 | 0.35 | 2.1 | <200 |
Percentage of CD4+ T cells that produce IFN-γ in fresh PBMC (day 0) and in culture (day 8).
Percentage of IFN-γ+ CD4+ T cells in the culture divided by the percentage in the fresh PBMC.
Copies of HIV-1 RNA/ml of culture supernatant.
ND, not determined.