Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2002 Jun;76(12):6364–6369. doi: 10.1128/JVI.76.12.6364-6369.2002

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2002, American Society for Microbiology

PMC Copyright notice

FIG. 2. — SMN and NS2 interact in vivo. (A) Anti-SMN (MANSMA3) coprecipitates endogenous SMN and hemagglutinin-tagged NS2 from A9_2L cells transiently expressing NS2. Anti-NS2 rabbit polyclonal sera were used to detect NS2. NS2 was not precipitated in the absence of MANSMA3 (−) or from nontransfected A9_2L cells (mock). mAb, monoclonal antibody; IP, immunoprecipitation. (B) BIA of NS2 coimmunoprecipitated with endogenous Smn from synchronized MVM-infected A9_2L cells (top panel). Total protein extracts from A9_2L cells 30 h postinfection were used. Anti-SMN (MANSMA3) coprecipitated SMN, and anti-NS2 rabbit polyclonal sera detected NS2 complexed with SMN. Captured levels of anti-SMN antibody (shift A), SMN/NS2 complex (shift B), and anti-NS2 rabbit polyclonal antibody (shift C) are indicated. NS2 is not coprecipitated with SMN from double-blocked, infected A9_2L cells 0 h postrelease (bottom panel). Total protein extracts were used from A9_2L cells 30 h after being released from the blocking process. Anti-SMN (MANSMA3)-precipitated SMN and anti-NS2 rabbit polyclonal sera detected no NS2 complexed with SMN. Captured levels of anti-SMN antibody (shift A), SMN (shift B), and anti-NS2 rabbit polyclonal antibody (shift C) are indicated as response units (RU).

FIG. 2. — SMN and NS2 interact in vivo. (A) Anti-SMN (MANSMA3) coprecipitates endogenous SMN and hemagglutinin-tagged NS2 from A9_2L cells transiently expressing NS2. Anti-NS2 rabbit polyclonal sera were used to detect NS2. NS2 was not precipitated in the absence of MANSMA3 (−) or from nontransfected A9_2L cells (mock). mAb, monoclonal antibody; IP, immunoprecipitation. (B) BIA of NS2 coimmunoprecipitated with endogenous Smn from synchronized MVM-infected A9_2L cells (top panel). Total protein extracts from A9_2L cells 30 h postinfection were used. Anti-SMN (MANSMA3) coprecipitated SMN, and anti-NS2 rabbit polyclonal sera detected NS2 complexed with SMN. Captured levels of anti-SMN antibody (shift A), SMN/NS2 complex (shift B), and anti-NS2 rabbit polyclonal antibody (shift C) are indicated. NS2 is not coprecipitated with SMN from double-blocked, infected A9_2L cells 0 h postrelease (bottom panel). Total protein extracts were used from A9_2L cells 30 h after being released from the blocking process. Anti-SMN (MANSMA3)-precipitated SMN and anti-NS2 rabbit polyclonal sera detected no NS2 complexed with SMN. Captured levels of anti-SMN antibody (shift A), SMN (shift B), and anti-NS2 rabbit polyclonal antibody (shift C) are indicated as response units (RU).