FIG. 6.
Comparison of the growth kinetics of recombinant and parental WNV. The growth of recombinant and parental viruses was compared at high and low MOIs on BHK-21 or Aedes albopictus C6/36 cells. (A) Growth in BHK-21 cells. (B) Growth in C6/36 cells. Viruses were inoculated at an MOI of 5.0 (filled symbols) or an MOI of 0.05 (open symbols) in triplicate in 12-well plates. Recombinant virus is designated by squares along a solid line. Parental virus is designated by triangles along a dashed line. Error bars represent ± standard deviation of triplicate wells. Dotted line indicates the limit of detection (500 PFU/ml).