FIG. 3.

Association of MiniRT2 with Hsp90 and p23 in mammalian cells. Plasmid DNA expressing the GST-tagged MiniRT2 (pEBG-MiniRT2) or GST alone (pEBG vector) was transfected into 293T cells. Transfected cells were lysed, and the GST proteins were purified by using GSH affinity beads. Bound proteins were eluted with GSH, resolved by SDS-PAGE, and detected by Western blot analyses using MAbs against Hsp90 (Anti-Hsp90) or p23 (Anti-p23) or by Coomassie blue staining (top panel). Hsp90, p23, GST-MiniRT2, and GST are indicated. The star to the left of the top panel denotes a nonspecific band associated with the GSH beads.