FIG. 4.

Triton X-114 phase separation of surface proteins from ST cells. The proteins recovered from the aqueous (a) and detergent (d) phases were electrophoretically separated and either visualized by silver staining (right panel) or, following transfer to nitrocellulose, analyzed for binding to the S protein of TGEV (left panel). The positions of molecular mass markers are indicated, as are the locations of aminopeptidase N and the high-molecular-mass sialoglycoprotein recognized by S (arrows).