FIG. 5.

Binding of TGEV to sialoglycoproteins from total cell lysates. Sialoglycoproteins were isolated from lysates of ST or LLC-PK1 cells by using wheat germ agglutinin-agarose. Mock-treated (−) and neuraminidase-treated (+) proteins were separated by SDS-PAGE under reducing conditions and transferred to nitrocellulose. The immobilized proteins were used for a virus overlay binding assay with neuraminidase-treated TGEV virions. Bound virus was visualized by an enzyme-linked immunoassay. The position of the high-molecular-mass sialoglycoprotein recognized by TGEV is indicated by an arrow.