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. 2026 Sep 16;10:292. doi: 10.1038/s41538-026-01162-2

Fig. 3. Effect of riboflavin on key indicators of arsenic-induced blood-testis barrier in mice.

Fig. 3

A, B Immunofluorescence staining and statistical analysis of Occludin (green) protein in testicular tissue, (Scale bars = 100 μm, n = 5). Nuclei were counterstained with DAPI (blue). C, D Immunofluorescence staining and statistical analysis of CX43 (red) protein in testicular tissue (Scale bars = 100 μm, n = 5). Nuclei were counterstained with DAPI (blue). E–H Western blotting analysis of the protein expression of Occludin, CX43, ZO-1 and N-Cadherin, respectively (n = 3). Note: Control: Control group; As: Arsenic trioxide group; Ribo: riboflavin group; As + Ribo: co-administration of arsenic trioxide and riboflavin group. ***P < 0.001, **P < 0.01 and *P < 0.05 vs. Control group; ###P < 0.001, ##P < 0.01 and #P < 0.05 vs. As group.